Sunday, September 23, 2012

Mitotic Phase of Onion (Allium cepa) [Lab. Activity]

When teaching students about the mitotic cell division, the most commonly used practice in secondary schools is the observation of the division phases of the onion root (Allium cepa). Here's my practicum recipe for the observation of onion root mitotic phase, along with the sample of results, data analysis of data and discussions. The observation in this article based on the observation that the author really did.


Tools and materials used
Tools:
Light Microscope
Set of Object Glass
Pipette
Tweezers
Watches glass
Rusty razor
Sharp razor
Vial bottle
Candles and lighters
Plastic and rubber

Materials:
Root tip of onion (Allium cepa)
Paper suction
Tissue
Alcohol 70%
FAA
HCl 1 N
Acetocarmin

Procedures:
Step 1: Preparation
First we must grow the root of onion, to grow the root, put the onion on the surface plastic glass with water. Pierce horizontally in the middle of onion, so that only the root touching the water. Let it grow in 1 week (7 days).  After the root growing, cut the tip of root, more or less 1 cm. Put the tip in vial bottle that was filled with a solution of FAA, then vial bottle sealed with plastic and tied with rubber.

Step 2: Execution
first thing to do is to make preparats, then continued with the observation the phases of mitosis under the microscope. To make the preparats, we must take the tip of onion root from vial bottle with tweezers  then put it on watch glass and pour with alcohol 70%. Let it be in 2 minutes.
After 2 min, remove  the alcohol 70% using suction paper, then add 1 N HCl solution then soak for 5 minutes. After 5 min, take the onion root from watch glass, cut the tip (root cap) and put it on a object glass.
The next step is drop acetocarmin on onion root and chopped with a rusty razor blade, followed by closed using a cover slip. Before observed under a microscope, preparats have to passed over the candle, then grinding it with your thumb or the blunt end of a pencil, then observed under a microscope.

Source
Anonim. 2004. The Cell Cycle & Mitosis Tutorial. (Online). http://www.biology. arizona.edu/cell_bio/tutorials/cell_cycle/cells3.html, diakses tanggal 28 Februari 2007

Margono, Hadi. 1973. Pengaruh Colchicine terhadap pertumbuhan Memanjang Akar Bawang Merah (Alium cepa). Skripsi tidak diterbitkan. Malang: IKIP

Setjo, Susetyoadi. 2004. Anatomi Tumbuihan. Malang: JICA.


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